Lattice light-sheet microscopy is a modified version of lightsheet fluorescence microscopy that increases image acquisition speed and resolution, while decreasing damage to cells caused by phototoxicity.

While classical lightsheet microscopy uses a static (SPIM) or scanned (DSLM) ligthsheet illumination with near Gaussian profile, the minimal thickness of the lightsheet (that determines axial resolution) and the usable Field of view are greatly limited by the Gaussian propagation limit. This can be addressed by ASLM (Axially-swept ligthsheet Mic.) though at the cost of speed.

Lattice lightsheet, instead, consists in creating a structured ligthsheet illumination field that will yield a thinner illumination extent together with a more extended usable Field of View. The lattice (patterned) lightsheet can be dithered to yield a uniform illumination, or rotated several times in a similar fashion to SIM.

Lattice lightsheet provides a superior combination of imaging speed, contrast and axial resolution, making it the most performant fluorescence imaging technique for fast live imaging at high resolution.