Red Española de Microscopía Óptica Avanzada
Optical Highlighters
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been engineered from existing fluorescent proteins or identified from large-scale screens in the wake of Kaede's discovery.
The classical PA-GFP converts from a non-radiant conformation to a GFP-like emitting fluorophore under UV excitation, while many of the other PAFPs undergo green-to-red photoconversions. Other colors are available. Some proteins take part in irreversible photoconversion reactions while other reactions can be reversed using light of a specific wavelength.
This technique at work
Universitat de Barcelona, CCiTUB- Centres Cientifics i Tecnologics - Advanced Optical Microscopy
Consejo Superior de Investigaciones Cientificas (CSIC), Centro de Biología Molecular Severo Ochoa - Servicio de Microscopia Optica y Confocal
Centro Andaluz de Nanomedicina y Biotechnología (BIONAND), Unidad de Nanoimagen - Advanced Optical Microscopy Service - Nanoimaging Unit
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
Instituto de Investigacion Biomedica Sant Pau, Instituto de Investigacion Sant Pau - Plataforma Cientifico-Tecnica de Microscopia Confocal
Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER, Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER - Microscopia
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