Red Española de Microscopía Óptica Avanzada
Photoactivation PA-FPs
 
Links
 
Optical Highlighters
 
Description
 
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been engineered from existing fluorescent proteins or identified from large-scale screens in the wake of Kaede's discovery.
The classical PA-GFP converts from a non-radiant conformation to a GFP-like emitting fluorophore under UV excitation, while many of the other PAFPs undergo green-to-red photoconversions. Other colors are available. Some proteins take part in irreversible photoconversion reactions while other reactions can be reversed using light of a specific wavelength.
This technique at work
 
Universitat de Barcelona, CCiTUB- Centres Cientifics i Tecnologics - Advanced Optical Microscopy
 
Consejo Superior de Investigaciones Cientificas (CSIC), Centro Nacional de Biotecnologia (CNB-CSIC) - Servicio de Microscopia Optica Avanzada del Centro Nacional de Biotecnologia
 
FPS-Fundacion Progreso y Salud, GENYO-Centro Pfizer-Universidad de Granada-Junta de Andalucia de Genomica e Investigacion Oncologica - Unidad de Microscopia e Imagen Molecular
 
Consejo Superior de Investigaciones Cientificas (CSIC), Centro de Biología Molecular Severo Ochoa - Servicio de Microscopia Optica y Confocal
 
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
 
Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER, Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER - Microscopia
 
 
Related training
 
 
Type Document Dates Lab
 
Related material
 
 
Type Details Title Lab