Cell biologists are increasingly using live-cell imaging techniques to
provide clues into the fundamental nature of cellular and tissue
structure and function. These investigations are being aided by the
explosive rate of developments in fluorescent protein, quantum dot, and
synthetic fluorophore technology. A number of technical challenges must
be overcome in order to perform successful live-cell imaging
experiments, including the ability to maintain cells in a healthy state
on the microscope stage for extended periods of time. New developments
in instrumentation (microscope systems, cameras, filter technology, and
illumination devices) have enabled imaging of a variety of dynamic
events with high spatial and temporal resolution over a wide range of
time scales.