Red Española de Microscopía Óptica Avanzada
Leica Leica TCS SP8 Laser Scanning Confocal Microscope
Universitat Jaume I, Servicio Central de Instrumentación Científica (SCIC) - Servicio Central de Instrumentación Científica
Lab contact
  • Universitat Jaume I
  • Servicio Central de Instrumentación Científica (SCIC)
 Av. de Vicent Sos Baynat, s/n, 12006 Castelló de la Plana, Castelló
Universitat Jaume I, Edificio de Investigación I
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Lab members
Lourdes Chiva Edo (Coordinadora SCIC)
Esther Irles
José A. Mata Martínez (Director SCIC)
Equipment description

Inverted Confocal Microscope Leica TCS SP8

  • The confocal light source consists on the following lasers: Diode 405, Argon (458, 488, 514), DPSS 561, HeNe 633.
  • There are 3 dry objectives: 5x, 10x, 20x; and 2 oil immersion objectives: 40x y 63x. The maximum of resolution with the 63x objective is 200nm in xy plane, and 500nm in z dimension. 
  • Fluorescence lamp Leica EL6000 (OSRAM HXP-R120W/45C VIS) to see from ocular lens.
  • Microscope techniques: bright field (BF), polarization (POL), differential interference contrast (DIC) and fluorescence (FLUO). These techniques can be combined at the same image.
  • Microscope fluorescence filters: DAPI, FITC, RHOD.
  • The software is called LAS X and allows to recover images 2D and 3D, spectral image, temporal image, and the combination of those.
Other info


  • CCD Microscope Camera DFC7000 T to capture the images we see from the ocular lens. The camera allows to combine several techniques (FLUO, DIC, etc.) and make a video with sequentially taken images.
  •  CO2 Microscope Cage Incubation System (okolab), to control the ‘in vivo’ conditions (temperature, CO2, O2) at the sample.
Technologies for this equipment
Laser Scanning Confocal Microscopy