Leica Leica TCS SP8 Laser Scanning Confocal Microscope
Universitat Jaume I, Servicio Central de Instrumentación Científica (SCIC) - Servicio Central de Instrumentación Científica
- Universitat Jaume I
- Servicio Central de Instrumentación Científica (SCIC)
Av. de Vicent Sos Baynat, s/n, 12006 Castelló de la Plana, Castelló
Universitat Jaume I, Edificio de Investigación I
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Lourdes Chiva Edo (Coordinadora SCIC) |
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Esther Irles |
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José A. Mata Martínez (Director SCIC) |
Equipment description
Inverted Confocal Microscope Leica TCS SP8
- The confocal light source consists on the following lasers: Diode 405, Argon (458, 488, 514), DPSS 561, HeNe 633.
- There are 3 dry objectives: 5x, 10x, 20x; and 2 oil immersion objectives: 40x y 63x. The maximum of resolution with the 63x objective is 200nm in xy plane, and 500nm in z dimension.
- Fluorescence lamp Leica EL6000 (OSRAM HXP-R120W/45C VIS) to see from ocular lens.
- Microscope techniques: bright field (BF), polarization (POL), differential interference contrast (DIC) and fluorescence (FLUO). These techniques can be combined at the same image.
- Microscope fluorescence filters: DAPI, FITC, RHOD.
- The software is called LAS X and allows to recover images 2D and 3D, spectral image, temporal image, and the combination of those.
Accessories:
- CCD Microscope Camera DFC7000 T to capture the images we see from the ocular lens. The camera allows to combine several techniques (FLUO, DIC, etc.) and make a video with sequentially taken images.
- CO2 Microscope Cage Incubation System (okolab), to control the ‘in vivo’ conditions (temperature, CO2, O2) at the sample.