Red Española de Microscopía Óptica Avanzada
Leica Microsystems TCS-SP2 Laser Scanning Confocal Microscope
IACS - Instituto Aragonés de Ciencias de la Salud, CIBA - Centro de Investigación Biomédica de Aragón - Servicio Cientifico-Tecnico de Microscopia y Anatomia Patologica
Lab contact
  • IACS - Instituto Aragonés de Ciencias de la Salud
  • CIBA - Centro de Investigación Biomédica de Aragón
Avda. San Juan Bosco, 13
Planta A
50009 Zaragoza
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Lab members
Maria Royo Cañas
Cesar Vallejo Ruiz
Equipment description

The Leica TCS SP2 is a spectral confocal with 3CH+DIC digital emission 400-800nm range designed to acquire high spatial resolution images of fluorescently labeled materials and for analysis of these images.

Our SP2 confocal system has seven laser excitation lines: 458, 476, 488, 496, 514, 543, and 633, all fiber coupled and controlled by AOTFs. The scan detector is equipped with an acousto-optical beam splitter (AOBS) to select/introduce most excitation laser lines, eliminating the need for main dichroic mirrors. The system has three PMT detectors with 8 or 12-bit output, simultaneous operation and digital/graphic definition of emission wavelength content per channel. Spectral scanning / display and linear un-mixing are possible, giving the ability to separate largely overlapping, similar colored fluorophores (e.g., GFP vs. FITC or YFP).

LCS Leica Confocal Software includes LCS 3D Volume rendering, 3D animations, 3D filter, Stereo images and animations, Average projection, Maximum point projection, SFP; LCS Multicolor Co-localization of 2channel and 3channel recordings. Cytofluorograms in 2D and 3D display. Histogram-segmentation and masking.

Use of this confocal system requires in-person training to make sure the system remains available to Users.

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Technologies for this equipment