Red Española de Microscopía Óptica Avanzada
Leica Laser Scanning Confocal TCS-SP5
 
Instituto de Investigacion Biomedica Sant Pau, Instituto de Investigacion Sant Pau - Plataforma Cientifico-Tecnica de Microscopia Confocal
 
Lab contact
 
 
Institution:
  • Instituto de Investigacion Biomedica Sant Pau
Center:
  • Instituto de Investigacion Sant Pau
 
Address
C/ Sant Antoni Maria Claret, 167, 08025 Barcelona
Antiguo Prefabricado de Historias Clinicas
Planta baja
 
 
view in Google Maps
 
view lab website
 
 
Lab members
 
 
Microscopia Sant Pau
 
 
 
Equipment description
 


Leica DMI 6000 CS inverted microscope coupled to a confocal system, with Filter A (blue), Filter I3 (green) and Filter N21 (red).

-Laser lines:
Argon; 458 nm, 476 nm, 488 nm, 496 nm, 514 nm.
DPSS; 561nm.
HeNe; 633nm.
Pulsed Lasers; 405nm, 470nm, 640nm.

-Scanners: galvano/resonant

-Objectives:
HCX PL APO lambda blue 63x/1.40-0.60 Oil
HCX PL APO CS 40x/1.25-0.75 Oil
HC PL APO CS 20x/0.70
HC PL APO CS 10x/0.40

-Mini-Incubation chamber for CO2 and temperature control

-Acquisition software: LAS-AF 2.5.1
 
Other info
 
 
Technologies for this equipment
 
FRET- Förster Resonance Energy Transfer
 
FLIM- Fluorescence Lifetime Imaging Microscopy
 
FCS- Fluorescence Correlation Spectroscopy
 
Brightfield & other Transmission Microscopy Contrasts
 
High Speed Laser Scanning Confocal Systems
 
Laser Scanning Confocal Microscopy
 
Live Cell Imaging
 
Image Analysis/ Software
 
Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photobleaching
 
Photoconversion