Red Española de Microscopía Óptica Avanzada
Nikon Eclipse Ti Brightfield/Transmission Microscopy Contrasts,Wide Field Fluorescence Microscope
 
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
 
Lab contact
 
 
Institution:
  • Instituto de Salud Carlos III
Center:
  • Centro Nacional de Investigaciones Cardiovasculares (CNIC)
 
Address
Fundacion CNIC Carlos III - Centro Nacional de Investigaciones Cardiovasculares
    Melchor Fernandez Almagro, 3.
    28029, Madrid. Spain.
 
 
view in Google Maps
 
view lab website
 
 
Lab members
 
 
Elvira Arza Cuesta
Valeria R. Caiolfa
Verónica Labrador Cantarero
Hélio Roque
Antonio Manuel Santos Beneit
Moreno Zamai
 
 
 
Equipment description
 
Epifluorescence in vivo time-lapse inverted microscope:

-Objectives:

     Plan Fluor 4x/0,13 PhL DL
     Plan Fluor 10x/0,3 Ph1 DLL
     S Plan Fluor ELWD 20x/0,45 Ph1 ADM
     Plan Apo λ 20x/0,75
     Plan Fluor ELWD 40x/0.6 Ph2 DM
     Plan Apo VC 60x/1,4 Oil DIC N2

-Fluorescence filters:

     DAPI: Exc 381-392nm; Em 447-507nm
     GFP: Exc 457-487nm; Em 502-538nm
     RED: Exc 542-582nm; Em 604-644nm
     Cy5: Exc 608-648nm; Em 672-712nm

-Cameras:

     Hamamatsu CCD ORCA-ER B/W
     ANDOR sCMOS ZYLA B/W

-Mini-incubator for CO2 and temperature control

-Acquisition software:  Nikon ACT-1 v 2.70
 
Other info
 
 
Technologies for this equipment
 
Brightfield & other Transmission Microscopy Contrasts
 
Live Cell Imaging
 
Wide Field Fluorescence Microscopy