Red Española de Microscopía Óptica Avanzada
Nikon A1R Laser Scanning Confocal Microscope
 
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
 
Lab contact
 
 
Institution:
  • Instituto de Salud Carlos III
Center:
  • Centro Nacional de Investigaciones Cardiovasculares (CNIC)
 
Address
Fundacion CNIC Carlos III - Centro Nacional de Investigaciones Cardiovasculares
    Melchor Fernandez Almagro, 3.
    28029, Madrid. Spain.
 
 
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view lab website
 
 
Lab members
 
 
Elvira Arza Cuesta
Valeria R. Caiolfa
Verónica Labrador Cantarero
Hélio Roque
Antonio Manuel Santos Beneit
Moreno Zamai
 
 
 
Equipment description
 
Nikon Eclipse-Ti inverted microscope coupled to a A1R confocal system:

-Laser lines:
     402,457,476,488,514,561 and 642nm

-4 PMT detectors with emission filters + 1 transmission detector

-Scanners: galvano/resonant

-Objectives:

     Plan Apo 10x/0,45 DIC L dry
     Plan Fluor EL WD 20x/0,45 DIC L dry
     Plan Apo VC 20x/0,75 DIC N2 dry
     Plan Fluor 40x/1,3 Oil DIC H N2 Oil
     Plan Apo VC 60x/1,4 Oil DIC N2

-Incubation chamber for CO2 and temperature control

-Acquisition software: NIS Elements AR 3.22.08 725
 
Other info
 
 
Technologies for this equipment
 
High Speed Laser Scanning Confocal Systems
 
Laser Scanning Confocal Microscopy
 
Live Cell Imaging
 
Photoactivation PA-FPs
 
Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photobleaching
 
Photoconversion