Red Española de Microscopía Óptica Avanzada
Leica Microsystems Laser Scanning Confocal TCS-SP5 Laser Scanning Confocal Microscope
 
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
 
Lab contact
 
 
Institution:
  • Instituto de Salud Carlos III
Center:
  • Centro Nacional de Investigaciones Cardiovasculares (CNIC)
 
Address
Fundacion CNIC Carlos III - Centro Nacional de Investigaciones Cardiovasculares
    Melchor Fernandez Almagro, 3.
    28029, Madrid. Spain.
 
 
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view lab website
 
 
Lab members
 
 
Elvira Arza Cuesta
Valeria R. Caiolfa
Verónica Labrador Cantarero
Hélio Roque
Antonio Manuel Santos Beneit
Moreno Zamai
 
 
 
Equipment description
 

Leica DMI6000B inverted microscope coupled to a confocal system:

-Laser lines:
     405,458,476,488,496,514,561,594 and 633nm

-5 spectral fluorescence detectors + 1 transmission detector

-Scanners: galvano/resonant

-Objectives:

     HCX PL APO CS 10x 0,4 dry
     HCX PL APO lambda blue 20x 0,7 multi-imm
     HCX PL APO CS 40x 1,25 oil
     HCX PL APO lambda blue 63x 1,2 water
     HCX PL APO CS 63 x 1,3 glycerol
     HCX PL APO lambda blue 63x 1,40 oil

-Incubation chamber for CO2 and temperature control

-Acquisition software: LAS-AF 2.6.0. build 7266

 
Other info
 
 
Technologies for this equipment
 
FRET- Förster Resonance Energy Transfer
 
High Speed Laser Scanning Confocal Systems
 
Laser Scanning Confocal Microscopy
 
Live Cell Imaging
 
Photoactivation PA-FPs
 
Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photobleaching
 
Photoconversion