Red Española de Microscopía Óptica Avanzada
Nikon Eclipse Ti Multiphoton Microscope
 
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
 
Lab contact
 
 
Institution:
  • Instituto de Salud Carlos III
Center:
  • Centro Nacional de Investigaciones Cardiovasculares (CNIC)
 
Address
Fundacion CNIC Carlos III - Centro Nacional de Investigaciones Cardiovasculares
    Melchor Fernandez Almagro, 3.
    28029, Madrid. Spain.
 
 
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view lab website
 
 
Lab members
 
 
Elvira Arza Cuesta
Valeria R. Caiolfa
Verónica Labrador Cantarero
Hélio Roque
Antonio Manuel Santos Beneit
Moreno Zamai
 
 
 
Equipment description
 


Customized Nikon Eclipse Ti inverted microscope coupled to a Multiphoton and FLIM system:

-MaiTai DS Laser. 690-1040 nm.

-2 PMT+ 2 APD detectors (1 ISS-fast-FLIM + 1 ISS- Fluorescence Correlation Spectroscopy)

-Objectives:
      Dry (40X/0.9; 40X/0.6)
      Water immersion (60X/1.2)
      Oil immersion (40X/0.9; 60X/1.2)

-Mini-incubator for CO2 and temperature control

-Acquisition software: VistaVision 4.0.119
 
Other info
 
 
Technologies for this equipment
 
FLIM- Fluorescence Lifetime Imaging Microscopy
 
Live Cell Imaging
 
Non-Linear Imaging/ Multiphoton fluorescence Imaging