Red Española de Microscopía Óptica Avanzada
Zeiss (Carl Zeiss Microscopy Gmbh) Multiphoton Laser Scanning Confocal Microscope,Multiphoton Microscope
 
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
 
Lab contact
 
 
Institution:
  • Instituto de Salud Carlos III
Center:
  • Centro Nacional de Investigaciones Cardiovasculares (CNIC)
 
Address
Fundacion CNIC Carlos III - Centro Nacional de Investigaciones Cardiovasculares
    Melchor Fernandez Almagro, 3.
    28029, Madrid. Spain.
 
 
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view lab website
 
 
Lab members
 
 
Elvira Arza Cuesta
Valeria R. Caiolfa
Verónica Labrador Cantarero
Hélio Roque
Antonio Manuel Santos Beneit
Moreno Zamai
 
 
 
Equipment description
 

Axio Examiner Z1 upright microscope coupled to a Confocal/Multiphoton/FLIM system with illumination sources for one and two photon excitation:

-Laser lines:
     405,458,488,514,561,633nm
     Mai Tai DS 690-1040 nm

-3 GASP detectors (1 spectral detector + 2 Becker&Hickl GmbH FLIM detectors in the NDD port) + 2PMTs + 1 Transmission detector.

-Objectives:
     W Plan-Apochromat 20x/1,0 DIC M27 75m
     W Plan-Apochromat 40x/1,0 DIC M27
      Plan-Apochromat 10x/0,45 M27

-Mini-incubator for CO2 and temperature control

-Acquisition software: ZEN 2011
 
Other info
 
 
Technologies for this equipment
 
FLIM- Fluorescence Lifetime Imaging Microscopy
 
FRET- Förster Resonance Energy Transfer
 
In vivo Imaging (animal)
 
Laser Scanning Confocal Microscopy
 
Live Cell Imaging
 
Non-Linear Imaging/ Multiphoton fluorescence Imaging
 
Non-Linear Imaging/ SHG-Second Harmonic Generation
 
Photoactivation PA-FPs
 
Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photobleaching
 
Photoconversion