Red Española de Microscopía Óptica Avanzada
Leica Microsystems Laser Scanning Confocal TCS-SP5 Multiphoton Microscope
Universitat Autonoma de Barcelona, Facultat de Ciencies - Servei de Microscopia
Lab contact
  • Universitat Autonoma de Barcelona
  • Facultat de Ciencies
Edifici C, Facultat de Ciencies
Campus de la UAB
08193 Bellaterra
Cerdanyola del Valles

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Lab members
Meritxell Vendrell
Equipment description

Inverted spectral confocal microscope equipped with 9 excitation lines, 6 detection channels (5 fluorescence/reflection, 1 transmitted light), with an AOBS® (Acousto Optical Beam Splitter) system. In vivo incubator  and a fast scanning module (resonant scanner).


Lasers:      Diode UV 405 nm (50mV)

                   Argon 458/476/488/496/514 nm

                   DPSS 561 nm

                   Helium-Neon 594 nm

                   Helium-Neon 633 nm


Fluorescence filters:          Excitation UV BP 340-380, emission LP 425

Excitation BP 405-490, emission LP 515

Excitation BP 515-560, emission LP 590


Detectors: 3 PMT + 1 HyD (hybrid detection system for photon counting)


Objectives:           HC PL APO CS 10x/0.40

HC PL APO CS 20x/0.70

PL APO 40x/1.25-0.75 CS oil

PL APO 63x/1.4-0.6 CS oil

PL APO 63x/1.2 W

PL APO 63x/1.3 Corr CS glicerol

HC PL Fluorar 50x/0.80 dry


Optics:          Bright field




In vivo system:        Microscope incubator (Life Imaging Services)

                                   Temperature controller

                                   CO2 controller

                                   Thermostatic and motorized/galvanometric plate


MP:         Titanium-Sapphire IR MaiTai broadband laser 710-990nm

2 external detectors for reflected light (NDD)

Other info
Technologies for this equipment