Red Española de Microscopía Óptica Avanzada
CUSTOM Laser Nanosurgery
IRB Barcelona (Institute for Research in Biomedicine), IRB Barcelona (Institute for Research in Biomedicine) - Advanced Digital Microscopy Core Facility
Lab contact
  • IRB Barcelona (Institute for Research in Biomedicine)
  • IRB Barcelona (Institute for Research in Biomedicine)
C. Baldiri Reixac, 10. 08028 Barcelona
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Lab members
Lídia Bardía
Julien Colombelli
Anna Lladó
Sébastien Tosi
Equipment description
A Pulsed Laser performing submicron ablation in cells and embryos, coupled onto an inverted microscope equipped with environmental control and spinning disk. Combination with FRAP also possible.

Multiscale performance: DNA Damage, subcellular ablation, organelles ablation, single cell ablation, cell scission, multicellular ablation, organ ablation, etc...

Applications: DNA damage kinetics, Cell biophysics, biomechanics, regeneration, morphogenesis, wound healing, tissue mechanics and development, micromanipulation, correlative microscopy LM-EM (laser etching to find samples) etc...
Other info
Most of experiments performed at 63x, water immersion NA = 1.2 lens,
possibility at different magnifications (40x 1.2W)

Laser specs: 355nm tripled Nd:YAG, pulse duration 470ps, rep rate up to 1kHz.
Technologies for this equipment