Red Española de Microscopía Óptica Avanzada
Leica Microsystems Laser Scanning Confocal TCS-SP5 Laser Scanning Confocal Microscope,Multiphoton Microscope
 
Centro Andaluz de Nanomedicina y Biotechnología (BIONAND), Unidad de Nanoimagen - Advanced Optical Microscopy Service - Nanoimaging Unit
 
Lab contact
 
 
Institutions:
  • Centro Andaluz de Nanomedicina y Biotechnología (BIONAND)
  • Universidad de Málaga
Center:
  • Unidad de Nanoimagen
 
Address
Centro Andaluz de Nanomedicina y Biotecnología (BIONAND)
Calle Severo Ochoa 35
Parque Tecnologico de Andalucia
Campanillas,
Malaga 29590 

 
 
view in Google Maps
 
view lab website
 
 
Lab members
 
 
John Pearson
 
 
 
Equipment description
 
Leica SP5 HyD MP Multiphoton Confocal Microscope
- Inverted Leica DMI 6000 fully motorised microscope.
- 4 x Conventional PMT detectors 
- 2 x External HyD Hybrid GaASP dedicated multiphoton detectors for the highest possible sensitivity.
- Tandem Scanner – HQ conventional scanner and high-speed resonant scanner 
- Microscope enclosure with temperature, humidity and CO2 control.
- High precision Super Z motorized stage with multi-point and multi-tile software control. 3nm resolution galvometric z-axis control

Objectives:
- Plan Apo Oil immersion: 100x (1.4 NA), 63x (1.4 NA Blue), 40x (1.3 NA) 
- Plan Apo 20x (0.7 NA) multi-immersion (water, glycerol, oil) 0.26mm WD
- Plan Apo 10x (0.4 NA) dry.
- Plan APO 25x (0.95 NA) water immersion, IR corrected for multiphoton, 2.2mm WD

Pulsed IR Laser for Multifoton
- Spectraphysics Titanium-Sapphire Mai-Tai Tunable Femtolaser.
- 690nm - 1040 nm range
- Control integrated into Leica software
- Suitable for multi-photon excitation and emission scans

Visible Laser lines:
- Violet 405nm (50mW), Blue Argon 458nm, 476nm, 488nm, 496nm, 514nm (65mW) Green DPSS 561nm (20mW), Orange HeNe 594nm (2mW), Red HeNe 633nm.





 
Other info
 

Access available to internal users, external academic users and companies.  Please contact Dr. John Pearson (+34 952 367 634 / jrpearson@bionand.es) for more information.