Red Española de Microscopía Óptica Avanzada
Super Resolution/ Stimulated Emission Depletion microscopy (STED)
 
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Description
 
STED (Stimulated Emission Depletion Microscopy) uses two laser pulses, the excitation pulse for excitation of the fluorophores to their fluorescent state and the STED pulse for the de-excitation of fluorophores by means of Stimulated emission. It allows one to achieve super resolution by selectively deactivating fluorophores in the vicinity of the focal spot, with engineered depletion laser beams, to reduce the emission and detection volumes and to enhance the final resolution of the laser-scanned image.
This technique at work
 
ICFO-Instituto de Ciencias Fotonicas, ICFO-Instituto de Ciencias Fotonicas - Servicio de Microscopia de Super-Resolucion y Nanoscopia- ICFO
 
Achucarro Basque Center for Neuroscience, - Imaging Facility
 
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
 
Centre for Genomic Regulation, Parc de Recerca Biomèdica Barcelona - Advanced Light Microscopy Unit
 
Consejo Superior de Investigaciones Cientificas (CSIC), Centro Nacional de Biotecnologia (CNB-CSIC) - Servicio de Microscopia Confocal del Centro Nacional de Biotecnologia
 
 
Related training
 
 
Type Document Dates Lab
CourseJORNADAS DE MICROSCOPÍA AVANZADA EN BIOCIENCIASdatesLabName
Course1st Practical School in Super-Resolution MicroscopydatesLabName
 
Related material
 
 
Type Details Title Lab