Red Española de Microscopía Óptica Avanzada
Super Resolution/ Stimulated Emission Depletion microscopy (STED)
STED (Stimulated Emission Depletion Microscopy) uses two laser pulses, the excitation pulse for excitation of the fluorophores to their fluorescent state and the STED pulse for the de-excitation of fluorophores by means of Stimulated emission. It allows one to achieve super resolution by selectively deactivating fluorophores in the vicinity of the focal spot, with engineered depletion laser beams, to reduce the emission and detection volumes and to enhance the final resolution of the laser-scanned image.
This technique at work
ICFO-Instituto de Ciencias Fotonicas, ICFO-Instituto de Ciencias Fotonicas - Servicio de Microscopia de Super-Resolucion y Nanoscopia- ICFO
Achucarro Basque Center for Neuroscience, - Imaging Facility
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
Centre for Genomic Regulation, Parc de Recerca Biomèdica Barcelona - Advanced Light Microscopy Unit
Consejo Superior de Investigaciones Cientificas (CSIC), Centro Nacional de Biotecnologia (CNB-CSIC) - Servicio de Microscopia Optica Avanzada del Centro Nacional de Biotecnologia
Related training
Type Document Dates Lab
Course1st Practical School in Super-Resolution MicroscopydatesLabName
Related material
Type Details Title Lab