Red Española de Microscopía Óptica Avanzada
Photobleaching Techniques/ FLIP-Fluorescence Loss In Photobleaching
 
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Description
 
This technique is used to study the diffusion of molecules among different connected/communicated cell compartments. A fluorescent cell is repeatedly photobleached within a small region while the whole cell is continuously imaged. Any regions of the cell that are connected to the bleached area will loose fluorescence due to the displacement of fluorescent proteins into this area. By contrast, the fluorescence in unconnected regions will not be affected.
This technique at work
 
Universitat de Barcelona, CCiTUB- Centres Cientifics i Tecnologics - Advanced Optical Microscopy Unit
 
Consejo Superior de Investigaciones Cientificas (CSIC), Centro Andaluz de Biologia del Desarrollo - Unidad de Microscopia Optica
 
FPS-Fundacion Progreso y Salud, GENYO-Centro Pfizer-Universidad de Granada-Junta de Andalucia de Genomica e Investigacion Oncologica - Unidad de Microscopia e Imagen Molecular
 
Universidad de Extremadura, Servicios de Apoyo a la Investigación - Servicio de Tecnicas Aplicadas a las Biociencias
 
Centro Andaluz de Nanomedicina y Biotechnología (BIONAND), Unidad de Nanoimagen - Advanced Optical Microscopy Service - Nanoimaging Unit
 
 
Related training
 
 
Type Document Dates Lab
 
Related material
 
 
Type Details Title Lab