Red Española de Microscopía Óptica Avanzada
Super Resolution/ PALM-Photoactivated Localization Microscopy
Photoactivated Localization Microscopy (PALM, or fPALM), is a super-resolution imaging technique that utilizes sequential activation and time-resolved localization of photoswitchable fluorophores to create high resolution images.  During imaging, only an optically resolvable subset of fluorophores is activated to a fluorescent state at any given moment, such that the position of each fluorophore can be determined with high precision by finding the centroid position of the single-molecule images. The fluorophore is subsequently deactivated, and another subset is activated and imaged. Iteration of this process allows numerous fluorophores to be localized and a super-resolution image to be constructed from the image data. PALM and fPALM were described using photoswitchable fluorescent proteins.
This technique at work
IMDEA Nanociencia, IMDEA Nanociencia - Laboratorio de AFM/Fluorescencia
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