Red Española de Microscopía Óptica Avanzada
Laser Microdissection & Catapulting
This technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A laser is coupled into a microscope and focuses onto the tissue on the slide. By movement of the laser by optics or the stage the focus follows a trajectory which is predefined by the user. This trajectory is then cut out and separated from the adjacent tissue. After the cutting process, an extraction process has to follow if an extraction process is desired. Ultraviolet pulsed lasers (355 nm) are commonly used for the cutting of the tissues and sometimes in combination with an IR laser responsible for heating/melting a sticky polymer for cellular adhesion and isolation.
This technique at work
FPS-Fundacion Progreso y Salud, GENYO-Centro Pfizer-Universidad de Granada-Junta de Andalucia de Genomica e Investigacion Oncologica - Unidad de Microscopia e Imagen Molecular
Consejo Superior de Investigaciones Cientificas (CSIC), Instituto de Biomedicina y Biotecnologia de Cantabria - IBBTEC - Servicio de Microscopia Optica Avanzada IBBTEC
Instituto de Salud Carlos III, Centro Nacional de Investigaciones Cardiovasculares (CNIC) - Microscopy and Dynamic Imaging Unit
Servicio de salud de Castilla-La Mancha (SESCAM), Hospital Nacional de Parapléjicos - Servicio de Microscopía y Análisis de Imagen
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