Red Española de Microscopía Óptica Avanzada
4Pi Microscopy
A 4Pi microscope is a laser scanning fluorescent microscope with an improved axial resolution. The typical value of 500–700 nm can be improved to 100–150 nm which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy.

The improvement in resolution is achieved by using two opposing objective lenses which both are focused to the same geometrical location.
This technique at work
CIB-Centro de Investigaciones Biológicas. CSIC, - TESTS from remoa webmaster
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