Red Española de Microscopía Óptica Avanzada
Laser Scanning Confocal Microscopy
Consejo Superior de Investigaciones Cientificas (CSIC), Centro de BiologĂ­a Molecular Severo Ochoa - Servicio de Microscopia Optica y Confocal
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical sectionsfrom thick specimens. The basic key to the confocal approach is the use of spatial filtering techniques to eliminate out-of-focus light or glare in specimens whose thickness exceeds the immediate plane of focus.
12 years
Equipment for this technology