Red Española de Microscopía Óptica Avanzada
Zeiss Axiovert 200M Wide Field Fluorescence Microscope
 
Consejo Superior de Investigaciones Cientificas (CSIC), Centro de Biología Molecular Severo Ochoa - Servicio de Microscopia Optica y Confocal
 
Lab contact
 
 
Institutions:
  • Consejo Superior de Investigaciones Cientificas (CSIC)
  • Universidad Autónoma de Madrid (UAM)
Center:
  • Centro de Biología Molecular Severo Ochoa
 
Address

+34 91 196 4643/4613. C/Nicolas Cabrera,1. Cantoblanco. 28049. Madrid

Red de Laboratorios de la Comunidad de Madrid

 
 
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view lab website
 
 
Lab members
 
 
Alfonsa Díaz
Fco. Javier Díez Guerra
Alejandro Molina
Ángeles Muñoz Alcalá
Carmen Sánchez Jiménez
Maite Villlalba Villacorta
 
 
 
Equipment description
 
Inverted Axiovert 200M motorized microscope with an EMCCD C9100-02 (Hamamatsu)
Motorized stage (Marzhauser)
High speed excitation and emission filter wheels (Sutter)
Incubation chamber for the microscope
Metamorph
 
Other info
 
  • Objectives (Zeiss)

- 10X/0.45 Plan-Apochromat Ph1 (BF, DIC II and Ph1)

- 20X/0.6 Plan-Apochromat Ph2 (BF, DIC II and Ph2)

- 40X/1.3 oil Plan-Neofluar Ph3 (Oil). (BF, DIC III and Ph3)

- 63X/1.4 oil Plan-Apochromat Ph3 (Oil) (BF, DIC III and Ph3)

- 63x/1.2 Water C-Apochromat Corr (Water) (BF and DICIII)

- 100X/1.45 oil Plan-Fluar (Oil) ((BF)

  • Fluorescence filters

- Cyan FP ( 426-446/422-432, 455 DCLP, 460-500)

- Yellow FP (490-510, 510-560, HQ520LP)

- Yellow FP BP (HQ 520-550/528.5-555.5)

- Dichroic CFP/YFP (440-500 510-610)

- GFP y DsRed (S473-498 S552-577 86007bs S500-540 S590-650)

- Rodamine (Em 560/40)

- Cy5 (HQ590-650, Q660LP, HQ662.5-737.5)

- Dapi (359-371, 450LP)
  • Optics

- Brightfield (BF)

- Nomarski (DIC)

- Phase contrast (Ph)

- Fluorescence

  • Digital camera

- C9100-02 (Hamamatsu)

  • Computer

- Display Mitsubishi 19 "

- Windows XP x32

- Metamorph 7.1 r2 (Universal Imaging)

  • Fluorescence

- High speed emission and excitation filter wheels: Sutter LAMBDA 10-2

- Motorized stage (Marzhauser)

- Xenon XBO 75W/2

  • Incubation system (Cell Observer-Zeiss) 

- Temperature control

- CO2 level control

- Humedity control

- Microscope incubator

- Perfusion system