Red Española de Microscopía Óptica Avanzada
Zeiss Multiphoton Laser Scanning Confocal Microscope,Multiphoton Microscope
 
Consejo Superior de Investigaciones Cientificas (CSIC), Centro de Biología Molecular Severo Ochoa - Servicio de Microscopia Optica y Confocal
 
Lab contact
 
 
Institutions:
  • Consejo Superior de Investigaciones Cientificas (CSIC)
  • Universidad Autónoma de Madrid (UAM)
Center:
  • Centro de Biología Molecular Severo Ochoa
 
Address

+34 91 196 4643/4613. C/Nicolas Cabrera,1. Cantoblanco. 28049. Madrid

Red de Laboratorios de la Comunidad de Madrid

 
 
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view lab website
 
 
Lab members
 
 
Alfonsa Díaz
Fco. Javier Díez Guerra
Alejandro Molina
Ángeles Muñoz Alcalá
Carmen Sánchez Jiménez
Maite Villlalba Villacorta
 
 
 
Equipment description
 
Confocal and Multiphoton system with two microscopes: 

1) LSM710 with an AxioObserver inverted microscope.
2 fluorescence detectors + 1 Quasar spectral detector + 1 transmission detector
2 Non-descanned detectors
Laser lines: 405/458/488/514/561/633/Infrared MaiTai Deep Sea
Incubation chamber for the microscope

2) LSM510 with an upright AxioImager M1.
1 fluorescence detector + 1 transmission detector
2 Non-descanned detectors
Laser lines: 543/Infrared MaiTai Deep Sea
 
Other info
 
  • Objectives

- 5x/0,16 EC "Plan-Neofluar" M27 (Brightfield)

- 10X/0.3 EC Plan-Neofluar M27 (Brightfield)

25x/0,8 LCI "Plan-Neofluar" Imm Corr DIC M27 (Oil.  Brightfield and DIC II)

- 20X/0.8 Plan-Apochromat (Brightfield and DIC II)

40x/1,30 EC "Plan-Neofluar" Oil DIC M27 (Oil.  Brightfield and DIC III)

- 63X/1.4 Plan-Apochromat Oil DIC M27 (Oil.  Brightfield and DIC III)

- 100x/1,40 Plan-Apochromat"  Oil DIC M27 (Oil.  Brightfield and DIC III)

40x/1,2 Water C-Apochromat" Corr UV-VIS-IR M27 (Water and immersion oil with RI 1.3. Brightfield and DIC III)

- 63x/1.2 Water C-Apochromat Corr UV-VIS-IR M27  (Water and immersion oil with RI 1.3. Brightfield and DIC III)

- 20x/0.5 Water N-Achroplan (Dipping. Brightfield and DIC II)

- 40x/1.0 Water Plan-Apochromat (Dipping. Brightfield and DIC III)

- 63x/1.0 Water Plan-Apochromat (Dipping. Brightfield and DIC III)

 

  • Fluorescence filters

- DAPI/GFP (Ex 395-420/475-500 Em 445-470/510-570) (51000 Chroma)

- CFP/DsRed (Ex 400-440/525-580 Em 460-510/590-710) (55018 Chroma)

 

  • Laser

- Diode 405 nm (on inverted microscope)

- Argon 458/488/514 nm (on inverted microscope)

- HeNe 543nm (on upright microscope)

- DPSS 561 nm (on inverted microscope)

- HeNe 633nm (on inverted microscope)

- MaiTai Deep Sea (Spectra Physics): 690-1020 nm, pulsos inferiores a 100 femtoseg, 2.5 Watt de potencia, 80 Mhz (on both microscopes)

 

  • Confocal filters

“Descanned” detection:

- Inverted microscope with spectral detector: Quasar + 2 PMTs

- Emission wavelenghts: 370-760 nm

 

- Upright microscope with these filters: BP 390-465, BP 435-485, BP 480-520, BP 500-550, BP 560-615, BP 650-710, KP 660

 

“Non-Descanned” detection:

- Inverted microscope: BP 380-430, BP 455-500, BP 465-515, BP 500-550, BP 520-560, BP 540-590, BP 565-610

- Upright microscope: BP 440-485, BP 455-500, BP 495-545, BP 500-550, BP 520-560, BP 570-640

 

  • Computer

- Windows Vista in the inverted microscope and XP in the upright one

- Software: Zeiss Zen2010B sp1 in the inverted microscope and Zen 2008 in the upright one

 

  • In Vivo incubation  system (Cell Observer-Zeiss)  (Inverted microscope)

- Perfusion system