Red Española de Microscopía Óptica Avanzada
Olympus FV1000 Total Internal Reflection Microscopy (TIRF)
 
Universitat Autonoma de Barcelona, Facultat de Ciencies - Servei de Microscopia
 
Lab contact
 
 
Institution:
  • Universitat Autonoma de Barcelona
Center:
  • Facultat de Ciencies
 
Address
Edifici C, Facultat de Ciencies
Campus de la UAB
08193 Bellaterra
Cerdanyola del Valles

 
 
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Lab members
 
 
Monica Roldan
Meritxell Vendrell
 
 
 
Equipment description
 

Inverted spectral confocal microscope equipped with 6 excitation lines, 4 detection channels (3 fluorescence/reflection, 1 transmitted light). An in vivo chamber can be attached.

 

Lasers:         Diode UV 405 nm (50 mV)

                      Argon 458/488/515 nm (30 mV)

                      Diode 560 nm (20 mV)

                      Diode 635 nm (20 mV)

 

Fluorescence filters:      Excitation UV BP 330-385, emission LP 420

                                           Excitation BP 460-490, emission SP 520

                                           Excitation BP 510-550, emission LP 510-550

 

Detectors: 3 PMT: 2 spectral detectors + 1 detection channel with filter

 

Objectives:           UPLSAPO10x / NA 0.40

                                UPLSAPO20x / NA 0.75

                                UPLFLN40xO / NA 1.3 oil

                                UPLSAPO60xO / NA 1.35 oil

                                PLAPON60xO / NA 1.45 oil (TIRF)

 

Optics:          Bright field

                      Nomarski

                      Phase contrast

 

TIRF illumination module + CellM software

 

In vivo system:           In vivo chamber (PeCon)

                                      Temperature controller

                                      CO2 controller

                                      Thermostatic plate

 

 
Other info
 
 
Technologies for this equipment