Red Española de Microscopía Óptica Avanzada
Leica Laser Scanning Confocal TCS-SP5 Multiphoton Microscope
 
Universitat Autonoma de Barcelona, Facultat de Ciencies - Servei de Microscopia
 
Lab contact
 
 
Institution:
  • Universitat Autonoma de Barcelona
Center:
  • Facultat de Ciencies
 
Address
Edifici C, Facultat de Ciencies
Campus de la UAB
08193 Bellaterra
Cerdanyola del Valles

 
 
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Lab members
 
 
Monica Roldan
Meritxell Vendrell
 
 
 
Equipment description
 

Inverted spectral confocal microscope equipped with 9 excitation lines, 6 detection channels (5 fluorescence/reflection, 1 transmitted light), with an AOBSŪ (Acousto Optical Beam Splitter) system. In vivo incubator  and a fast scanning module (resonant scanner).

 

Lasers:      Diode UV 405 nm (50mV)

                   Argon 458/476/488/496/514 nm

                   DPSS 561 nm

                   Helium-Neon 594 nm

                   Helium-Neon 633 nm

 

Fluorescence filters:          Excitation UV BP 340-380, emission LP 425

Excitation BP 405-490, emission LP 515

Excitation BP 515-560, emission LP 590

 

Detectors: 3 PMT + 1 HyD (hybrid detection system for photon counting)

 

Objectives:           HC PL APO CS 10x/0.40

HC PL APO CS 20x/0.70

PL APO 40x/1.25-0.75 CS oil

PL APO 63x/1.4-0.6 CS oil

PL APO 63x/1.2 W

PL APO 63x/1.3 Corr CS glicerol

HC PL Fluorar 50x/0.80 dry

 

Optics:          Bright field

                      Nomarski

                      Polarized

 

In vivo system:        Microscope incubator (Life Imaging Services)

                                   Temperature controller

                                   CO2 controller

                                   Thermostatic and motorized/galvanometric plate

 

MP:         Titanium-Sapphire IR MaiTai broadband laser 710-990nm

2 external detectors for reflected light (NDD)

 
Other info
 
 
Technologies for this equipment