Red Española de Microscopía Óptica Avanzada
Leica TCS SP8 Laser Scanning Confocal Microscope
UPM- Universidad Politecnica de Madrid, CBGP- Centro de Biotecnologia y Genomica de Plantas - CBGP Microscopy service
Lab contact
  • UPM- Universidad Politecnica de Madrid
  • INIA- Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria
  • CBGP- Centro de Biotecnologia y Genomica de Plantas
Centro de Biotecnologia y Genomica de Plantas UPM-INIA
Parque Cientifico y Tecnologico de la UPM
Campus de Montegancedo
28223 Pozuelo de Alarcon (Madrid)
Tel.: +34 913364530
Fax: +34 917157721
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Lab members
Pablo Gonzalez-Melendi de León
Equipment description
Inverted microscope Leica DMI6000CS

Touchscreen STP6000
Metal halide lamp EL6000 (120 W)

Fluorescence cubes:
Type Light          Excitation Dichroic Emission
A4 UV              BP 360/40 400 BP 470/40
CFP violet/blue   BP 436/20 455 BP 480/40
I3 blue BP 450-490 510 LP 515
N2.1 green BP 515-560 580 LP 590

Type                 Magnification/NA  Technique  Immersion
PL APO CS      10x/0.4       
PL APO CS      20x/0.70       
PL APO CS2    20x/0.75               Nomarski   water, glycerol, oil
PL APO CS2    40x/1.1                 Nomarski   water
PL APO CS2    63x/1.4                 Nomarski   oil

Ultraspectral confocal module Leica TCS SP8

FOV scanning: speed up to 1.800 Hz (3.600 Hz in bidirectional mode), 7 fps @ 512 x 512 px, resolution up to 8.192 x 8.192 px.
4 spectral detectors: 2 PMT and 2 HyD
1 detector for transmitted light

LIAchroic beam splitter, which includes the dichroic mirrors:
substrate (405)
RT 15/85 (reflection)

CW (405 nm). 50 mW
Ar (458, 488 and 514 nm). 100 mW
DPSS (561 nm). 20 mW.
He-Ne (633nm). 10 mW

LAS AF SP8 microlab with wizard for FRET & FRAP analysis

Other info
Technologies for this equipment