Red Española de Microscopía Óptica Avanzada
Leica TCS SP8 Laser Scanning Confocal Microscope
 
UPM- Universidad Politecnica de Madrid, CBGP- Centro de Biotecnologia y Genomica de Plantas - CBGP Microscopy service
 
Lab contact
 
 
Institutions:
  • UPM- Universidad Politecnica de Madrid
  • INIA- Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria
Center:
  • CBGP- Centro de Biotecnologia y Genomica de Plantas
 
Address
Centro de Biotecnologia y Genomica de Plantas UPM-INIA
Parque Cientifico y Tecnologico de la UPM
Campus de Montegancedo
28223 Pozuelo de Alarcon (Madrid)
Tel.: +34 913364530
Fax: +34 917157721
 
 
view in Google Maps
 
view lab website
 
 
Lab members
 
 
Pablo Gonzalez-Melendi
Beatriz Mora Rojas
 
 
 
Equipment description
 
Inverted microscope Leica DMI6000CS

Touchscreen STP6000
Metal halide bulb EL6000 (120 W)

Fluorescence cubes:
Type    Light          Excitation    Dichroic   Emission
A4       UV            BP 360/40    400         BP 470/40
CFP    violet/blue   BP 436/20    455         BP 480/40
I3        blue           BP 450-490   510        LP 515
N2.1    green         BP 515-560   580        LP 590

Objectives:
Type                 Magnification/NA  Technique  Immersion
PL APO CS      10x/0.4       
PL APO CS      20x/0.70       
PL APO CS2    20x/0.75               Nomarski   water, glycerol, oil
PL APO CS2    40x/1.1                 Nomarski   water
PL APO CS2    63x/1.4                 Nomarski   oil

Ultraspectral confocal module Leica TCS SP8

FOV scanning: speed up to 1.800 Hz (3.600 Hz in bidirectional mode), 7 fps @ 512 x 512 px, resolution up to 8.192 x 8.192 px.
3 spectral confocal detectors (PMT) for fluorescence and reflection (one of them of the highest sensitivity, HyD), and 1 detector for transmitted light (Nomarski)

LIAchroic beam splitter, which includes the dichroic mirrors:
substrate (405)
488/561/633
458/514/561
476/488
RT 15/85 (reflection)
488/561

Lasers:
CW (405 nm). 50 mW
Ar (458, 488 and 514 nm). 100 mW
DPSS (561 nm). 20 mW.
He-Ne (633nm). 10 mW

Software
LAS AF SP8 microlab with wizard for FRET & FRAP analysis

 
Other info
 
 
Technologies for this equipment