A Pulsed Laser performing submicron ablation in cells and embryos, coupled onto an inverted microscope equipped with environmental control and spinning disk. Combination with FRAP also possible.
Multiscale performance: DNA Damage, subcellular ablation, organelles ablation, single cell ablation, cell scission, multicellular ablation, organ ablation, etc...
Applications: DNA damage kinetics, Cell biophysics, biomechanics, regeneration, morphogenesis, wound healing, tissue mechanics and development, micromanipulation, correlative microscopy LM-EM (laser etching to find samples) etc...
Most of experiments performed at 63x, water immersion NA = 1.2 lens,
possibility at different magnifications (40x 1.2W)
Laser specs: 355nm tripled Nd:YAG, pulse duration 470ps, rep rate up to 1kHz.