Lightsheet based Macroscope to image full organ and tumors after optical clearing.
The system is based on Low magnification Imaging (macroscope) and achieves isotropic detection within a zoom range of 1x-20x. Image tiling working well to generate typical 20x20x3000 images of large organs at higher magnification for single cell detection in organs.
Currently compatible with the following clearing techniques tested in the lab:
Dehydration + BABB, Dehydration + DBE, iDisco, 3Disco, CUBIC, Rapiclear.
Optical zoom from 1.2x to 25x.
Lightsheet thickness from 4 to 20 microns.
Orca R2 CCD camera, Hamamatsu.
Lasers: 405, 488, 561, 638nm.
Full Multiposition automation, custom Labview control.