Red Española de Microscopía Óptica Avanzada
CUSTOM Macro-SPIM Single Plane Illumination Microscope (SPIM)
 
IRB Barcelona (Institute for Research in Biomedicine), IRB Barcelona (Institute for Research in Biomedicine) - Advanced Digital Microscopy Core Facility
 
Lab contact
 
 
Institution:
  • IRB Barcelona (Institute for Research in Biomedicine)
Center:
  • IRB Barcelona (Institute for Research in Biomedicine)
 
Address
C. Baldiri Reixac, 10. 08028 Barcelona
 
 
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view lab website
 
 
Lab members
 
 
Lídia Bardía
Julien Colombelli
Anna Lladó
Sébastien Tosi
 
 
 
Equipment description
 
Lightsheet based Macroscope to image full organ and tumors after optical clearing.
The system is based on Low magnification Imaging (macroscope) and achieves isotropic detection within a zoom range of 1x-20x.  Image tiling working well to generate typical 20x20x3000 images of large organs at higher magnification for single cell detection in organs.

Currently compatible with the following clearing techniques tested in the lab:
Dehydration + BABB, Dehydration + DBE, iDisco, 3Disco, CUBIC, Rapiclear.
 
Other info
 
Optical zoom from 1.2x to 25x.
Lightsheet thickness from 4 to 20 microns.
Orca R2 CCD camera, Hamamatsu.
Lasers: 405, 488, 561, 638nm.
Full Multiposition automation, custom Labview control.
 
Technologies for this equipment
 
Single Plane Illumination Microscopy (SPIM)