Red Española de Microscopía Óptica Avanzada
CUSTOM SPIM Single Plane Illumination Microscope (SPIM)
 
IRB Barcelona (Institute for Research in Biomedicine), IRB Barcelona (Institute for Research in Biomedicine) - Advanced Digital Microscopy Core Facility
 
Lab contact
 
 
Institution:
  • IRB Barcelona (Institute for Research in Biomedicine)
Center:
  • IRB Barcelona (Institute for Research in Biomedicine)
 
Address
C. Baldiri Reixac, 10. 08028 Barcelona
 
 
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Lab members
 
 
Lídia Bardía
Julien Colombelli
Anna Lladó
Sébastien Tosi
 
 
 
Equipment description
 
Lightsheet Microscope for long-term and multi-view Imaging of large specimens.
Typical Applications: embryo development, 3D cell cultures, intravital microscopy, transparent organisms imaging, cultured cells on glass coverslip to be imaged "fast" and at different angles.
 
Other info
 
sCMOS Hamamatsu Flash4.0 camera.
Fast piezo scan for the z-axis.
Imaging of samples embedded in Agarose blocks or on coverslip.
Large chamber with medium circulation.
Double sided illumination, Pivot-scanning under construction.
Lasers: 405nm, 488n,, 561nm, 638nm.
Water dipping objective lenses: 10x, 20x, 40x, 63x.
 
Technologies for this equipment
 
Single Plane Illumination Microscopy (SPIM)