Red Española de Microscopía Óptica Avanzada
Nikon Ti Total Internal Reflection Microscopy (TIRF)
 
Centro Andaluz de Nanomedicina y Biotechnología (BIONAND), Unidad de Nanoimagen - Advanced Optical Microscopy Service - Nanoimaging Unit
 
Lab contact
 
 
Institutions:
  • Centro Andaluz de Nanomedicina y Biotechnología (BIONAND)
  • Universidad de Málaga
Center:
  • Unidad de Nanoimagen
 
Address
Centro Andaluz de Nanomedicina y Biotecnología (BIONAND)
Calle Severo Ochoa 35
Parque Tecnologico de Andalucia
Campanillas,
Malaga 29590 

 
 
view in Google Maps
 
view lab website
 
 
Lab members
 
 
John Pearson
 
 
 
Equipment description
 
Inverted Nikon microscope for laser-based TIRF and N-STORM super-resolution microscopy

- Motorised TIRF mirror 
- Multipass TIRF dichroic filter (allows high-speed multicolour TIRF)
- Andor iXon3 897 EM-CCD camera 
- Microscope enclosure with temperature, humidity and CO2 control
- High precision motorized stage
- Nikon PFS (Perfect Focus System) for improved stability

N-STORM Super-resolution
- Multipass N-Storm filter allowing fast excitation of Reporter (Alexa647) and multiple Activator (e.g. Alexa405, Cy2 and Cy3) fluorochromes.
-  Integrated acquisition and analysis modules capable of multicolor super-resolution imaging up to 20nm lateral resolution.
- dSTORM compatible 
- Cylindrical lens allowing 3D super-resolution point mapping up to 60nm axially.
- Additional workstation with N-STORM module for offline image processing

Objective lenses:
o Plan Apo Oil immersion: 100x (1.49 NA) TIRF o Plan Fluor 40x (0.75 NA)
o Plan Apo 10x (0.45 NA).

Lasers

- Violet 405nm
- Argon-Ion 457nm, 477nm, 488nm, 514nm
- Yellow Diode 561nm
- High power Red 647nm (300mW) for N-STORM

 
Other info
 

Access available to internal users, external academic users and companies.  Please contact Dr. John Pearson (+34 952 367 634 / jrpearson@bionand.es) for more information.