Red Española de Microscopía Óptica Avanzada
Nikon Ti Total Internal Reflection Microscopy (TIRF)
Centro Andaluz de Nanomedicina y Biotechnología (BIONAND), Unidad de Nanoimagen - Advanced Optical Microscopy Service - Nanoimaging Unit
Lab contact
  • Centro Andaluz de Nanomedicina y Biotechnología (BIONAND)
  • Universidad de Málaga
  • Unidad de Nanoimagen
Centro Andaluz de Nanomedicina y Biotecnología (BIONAND)
Calle Severo Ochoa 35
Parque Tecnologico de Andalucia
Malaga 29590 

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Lab members
John Pearson
Equipment description
Inverted Nikon microscope for laser-based TIRF and N-STORM super-resolution microscopy

- Motorised TIRF mirror 
- Multipass TIRF dichroic filter (allows high-speed multicolour TIRF)
- Andor iXon3 897 EM-CCD camera 
- Microscope enclosure with temperature, humidity and CO2 control
- High precision motorized stage
- Nikon PFS (Perfect Focus System) for improved stability

N-STORM Super-resolution
- Multipass N-Storm filter allowing fast excitation of Reporter (Alexa647) and multiple Activator (e.g. Alexa405, Cy2 and Cy3) fluorochromes.
-  Integrated acquisition and analysis modules capable of multicolor super-resolution imaging up to 20nm lateral resolution.
- dSTORM compatible 
- Cylindrical lens allowing 3D super-resolution point mapping up to 60nm axially.
- Additional workstation with N-STORM module for offline image processing

Objective lenses:
o Plan Apo Oil immersion: 100x (1.49 NA) TIRF o Plan Fluor 40x (0.75 NA)
o Plan Apo 10x (0.45 NA).


- Violet 405nm
- Argon-Ion 457nm, 477nm, 488nm, 514nm
- Yellow Diode 561nm
- High power Red 647nm (300mW) for N-STORM

Other info

Access available to internal users, external academic users and companies.  Please contact Dr. John Pearson (+34 952 367 634 / for more information.